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Anti -S100A9 Rabbit pAb

價格: 在線咨詢
貨號:
KT012261
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
M/R,M/R,M/R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

S100A9 rabbit polyclonal

Protein full name

Protein S100-A9

Synonyms

Calgranulin-B, Leukocyte L1 complex heavy chain, MRP-14, p14, S100 calcium-binding protein A9, S100a9, Cagb, Mrp14

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse S100A9

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Cell membrane, Secreted

Predicted MW.

13 kDa

Observed MW.

13 kDa

Uniprot ID

P31725, P50116




Applications



Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 300-1: 800

spleen, lung

IHC

Mouse, Rat

1: 1500-1: 3000

lung, liver, spleen, small intestine

IF

Mouse, Rat

1: 750-1: 1500

lung, spleen





Background


Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.




Images


Western blot analysis of S100A9 (GB111149) at dilution of 1: 800
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse lung tissue lysate
Lane 1: Rat spleen tissue lysate
Lane 2: Rat lung tissue lysate

Immunohistochemistry analysis of paraffin-embedded mouse lung using S100A9 at dilution of 1: 1800

Immunohistochemistry analysis of paraffin-embedded rat lung using S100A9 (GB111149) at dilution of 1: 1800

Immunohistochemistry analysis of paraffin-embedded mouse liver using S100A9 (GB111149) at dilution of 1: 1800


Immunohistochemistry analysis of paraffin-embedded rat liver using S100A9 (GB111149) at dilution of 1: 1800


Immunohistochemistry analysis of paraffin-embedded mouse spleen using S100A9 (GB111149) at dilution of 1: 1800

Immunohistochemistry analysis of paraffin-embedded rat spleen using S100A9 (GB111149) at dilution of 1: 1800

Immunohistochemistry analysis of paraffin-embedded rat small intestine using S100A9 (GB111149) at dilution of 1: 1800

Immunofluorescent analysis of paraffin-embedded mouse lung using S100A9 (GB111149) at dilution of 1: 1000

Immunofluorescent analysis of paraffin-embedded mouse spleen using S100A9 (GB111149) at dilution of 1: 1000

Immunofluorescent analysis of paraffin-embedded rat spleen using S100A9 (GB111149) at dilution of 1: 1000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.
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