Anti -EIF2S2/EIF2B Rabbit pAb

價(jià)格：在線咨詢

貨號：

KT012119

產(chǎn)品規(guī)格：

100 μL

Applications：

WB/IHC/IF

Species：

H/M/R,H/M/R,H/M/R

品牌：

萬物

購買數(shù)量：

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產(chǎn)品詳情操作說明注意事項(xiàng)

Product Information

Description	EIF2S2 rabbit polyclonal
Protein full name	Eukaryotic translation initiation factor 2 subunit 2
Synonyms	eIF-2-beta, Eif2s2, Eukaryotic translation initiation factor 2 subunit beta
Immunogen	Recombinant protein corresponding to Mouse EIF2S2
Isotype	IgG
Purity	Affinity purification
Subcellular location	Cytoplasm
Predicted MW.	38 kDa
Observed MW.	50 kDa
Uniprot ID	P20042, Q99L45

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	lung, colon, small intestine
IHC	Human, Mouse, Rat	1: 1000-1: 2000	colon, lung, placenta
IF	Human, Mouse, Rat	1: 500-1: 3000	colon, brain

Background

Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.

Images

	Western blot analysis of EIF2S2 (GB111135) at dilution of 1: 1000 Lane 1: HT29 cell lysate Lane 2: L1299 cell lysate Lane 3: CaCO2 cell lysate Lane 4: Mouse lung tissue lysate Lane 5: Mouse colon tissue lysate Lane 6: Mouse small intestine tissue lysate Lane 7: Rat lung tissue lysate
	Immunohistochemistry analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000
	Immunohistochemistry analysis of paraffin-embedded rat lung using EIF2S2 (GB111135) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded rat placenta using EIF2S2 (GB111135) at dilution of 1: 1000
	Immunofluorescent analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000
	Immunofluorescent analysis of paraffin-embedded rat brain using EIF2S2 (GB111135) at dilution of 1: 1000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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