Anti -Heme-regulated inhibitor Rabbit pAb

價格：在線咨詢

貨號：

KT012021

產(chǎn)品規(guī)格：

100ul

Applications：

WB/IHC/IF

Species：

H/M/R,M/R,M/R

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項

Product Information

Description	Heme-regulated inhibitor rabbit polyclonal
Protein full name	Eukaryotic translation initiation factor 2-alpha kinase 1
Synonyms	Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Eif2ak1, Heme-controlled repressor, HCR, Heme-regulated inhibitor, Hri
Immunogen	Recombinant protein corresponding to Mouse Heme-regulated inhibitor
Isotype	IgG
Purity	Affinity purification
Predicted MW.	70 kDa
Observed MW.	70 kDa
Subcellular location	Cytoplasm
Uniprot ID	Q9BQI3, Q9Z2R9, Q63185

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	HEK293
IHC	Mouse, Rat	1: 800-1: 1600	bone, lung, inflammation lung
IF	Mouse, Rat	1: 500-1: 1000	bone, lung, inflammation lung

Background

The Eif2ak1 Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells, controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties.

Images

	Western blot analysis of Eif2ak1 (GB111094) at dilution of 1: 1000 Lane 1: HEK293 cell lysate
	Immunohistochemistry analysis of paraffin-embedded mouse bone using Eif2ak1 (GB111094) at dilution of 1: 1000
	Immunohistochemistry analysis of paraffin-embedded rat lung using Eif2ak1 (GB111094) at dilution of 1: 1000
	Immunohistochemistry analysis of paraffin-embedded mouse inflammation lung using Eif2ak1 (GB111094) at dilution of 1: 1000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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