Anti -Cyclin E1 Rabbit pAb

價(jià)格：在線咨詢

貨號：

KT011860

產(chǎn)品規(guī)格：

100 μL

Applications：

WB | IHC

Species：

H,M,R | H,M,R

品牌：

萬物

購買數(shù)量：

立即詢價(jià) 在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項(xiàng)

Product Information

Description	Cyclin E1 rabbit polyclonal
Protein full name	G1/S-specific cyclin-E1
Synonyms	CCNE1, CCNE, pcyclin E1, Cyclin E1
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse Cyclin E1
Isotype	IgG
Purity	Affinity purification
Subcellular location	Nucleus
Predicted MW.	47 kDa
Observed MW.	47 kDa
Uniprot ID	P24864, Q61457

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human, Mouse, Rat	1: 200-1: 400	testis, colon, placenta
IHC	Human, Mouse, Rat	1: 100-1: 400	placenta, colon, testis

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Images

	Western blot analysis of Cyclin E1 (GB111936) at dilution of 1: 400 Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: K562 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Mouse colon tissue lysate Lane 6: Rat testis tissue lysate Lane 7: Rat placenta tissue lysate
	Immunohistochemical analysis of hyperbaric repair of paraffin-embedded human placenta using Cyclin E1 (GB111936) at dilution of 1: 200
	Immunohistochemical analysis of hyperbaric repair of paraffin-embedded human colon using Cyclin E1(GB111936) at dilution of 1: 200
	Immunohistochemical analysis of hyperbaric repair of paraffin-embedded mouse testis using Cyclin E1(GB111936) at dilution of 1: 200

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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