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Anti -14-3-3 zeta Rabbit pAb
價格：
在線咨詢
貨號：
KT011693
產(chǎn)品規(guī)格：
100 μL
Applications：
WB | IHC | IF
Species：
H,M,R | M,R | M,R
購買數(shù)量：
產(chǎn)品詳情
操作說明
注意事項
Product Information
|
Description |
14-3-3 zeta rabbit polyclonal |
|
Protein full name |
14-3-3 protein zeta/delta |
|
Synonyms |
Protein kinase C inhibitor protein 1, KCIP-1, SEZ-2, Ywhaz, 14-3-3 Delta, 14-3-3 Zeta |
|
Immunogen |
Recombinant protein corresponding to Mouse 14-3-3 zeta |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
|
|
Predicted MW. |
28 kDa |
|
Observed MW. |
28 kDa |
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
lung, kidney, brain |
|
IHC |
Mouse, Rat |
1: 1000-1: 2000 |
brain |
|
IF |
Mouse, Rat |
1: 500-1: 1000 |
brain |
Background
YWHAZ belongs to the 14-3-3 family of proteins which are in charge for checkpoint control, apoptotic & nutrient sensing pathways as well as signal transduction by binding to phosphoserine-containing proteins. This highly conserved and ubiquitously expressed protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. It interacts with IRS1 protein,signifying a role in regulating insulin. YWHAZ function as an adapter protein involved in the regulation of a large spectrum of both general and specialized signaling pathway. YWHAZ binds to a large number of partners,usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Images
|
|
Western blot analysis of 14-3-3 zeta (GB111023) at dilution of 1: 1000 Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Mouse lung tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat lung tissue lysate Lane 8: Rat kidney tissue lysate Lane 9: Rat brain tissue lysate |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse brain using 14-3-3 zeta (GB111023) at dilution of 1: 2000 |
|
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Immunohistochemistry analysis of paraffin-embedded rat brain using 14-3-3 zeta (GB111023) at dilution of 1: 2000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學(xué)科技園A401室