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Anti -PHD2 / prolyl hydroxylase Rabbit pAb
價(jià)格：
在線咨詢
貨號(hào)：
KT012020
產(chǎn)品規(guī)格：
100 μL
Applications：
IHC/IF
Species：
MR/MR
購(gòu)買(mǎi)數(shù)量：
產(chǎn)品詳情
操作說(shuō)明
注意事項(xiàng)
Product Information
|
Description |
PHD2 rabbit polyclonal |
|
Protein full name |
Egl nine homolog 1 |
|
Synonyms |
Hypoxia-inducible factor prolyl hydroxylase 2, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, Prolyl hydroxylase domain-containing protein 2, PHD2, SM-20, Egln1 |
|
Immunogen |
Recombinant protein corresponding to Mouse PHD2 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm. Nucleus |
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
IHC |
Mouse, Rat |
1: 850-1: 2000 |
brain, heart |
|
IF |
Mouse, Rat |
1: 500-1: 1000 |
brain, heart |
Background
Prolyl hydroxylase 2(PHD2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402), Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex.
Images
|
|
Immunohistochemistry analysis of paraffin-embedded mouse brain using PHD2 (GB111030) at dilution of 1: 1700 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse heart using PHD2 (GB111030) at dilution of 1: 1700 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat brain using PHD2 (GB111030) at dilution of 1: 1700 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥萬(wàn)物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室