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Recombinant Anti - beta Actin antibody ( Rabbit mAb )

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貨號:
DRF1013
產(chǎn)品規(guī)格:
100 μL
Applications:
WB | IHC/IF | ICC/IF
Species:
H,R,M | H,R,M | H,R,M
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

Mouse monoclonal to beta III Tubulin

Protein full name

Tubulin beta-3 chain

Synonyms

TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III, β3- tubulin

Immunogen

KLH conjugated synthetic peptide

corresponding to Mouse beta III- Tubulin

Isotype

IgG1

Purity

Affinity purification

Subcellular location

CytoplasmCytoskeleton

Predicted MW.

55 kDa

Observed MW.

55 kDa

Uniprot ID

Q13509, Q9ERD7, Q4QRB4




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1:2000

brain, cerebral cortex

IHC

Human, Mouse, Rat

1: 600-1: 1200

cerebellum

IF

Human, Mouse, Rat

1: 600-1: 1200

cerebellum




Background


Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response.This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.




Images


Western blot analysis of beta III- Tubulin (GB15139) at dilution of 1: 1000

Immunohistochemistry analysis of paraformaldehyde-fixed mouse cerebellum using beta III- Tubulin (GB15139) at dilution of 1: 1800




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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