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Anti -160 kDa Neurofilament Medium Rabbit pAb

價格: 在線咨詢
貨號:
KT011891
產(chǎn)品規(guī)格:
100ul
Applications:
WB|IHC/IF
Species:
H/M/R,M/R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

160 kDa Neurofilament Medium rabbit polyclonal antibody

Protein full name

Neurofilament medium polypeptide

Synonyms

160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nef3, Nefm, NF-M, Nfm

Immunogen

Recombinant protein corresponding to Mouse 160 kDa Neurofilament Medium

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Cytoskeleton

Predicted MW.

96 kDa

Observed MW.

150 kDa

Uniprot ID

P07197, P08553, P12839




Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 500-1: 1000

brain, spinal cord

IHC/IF

Mouse, Rat

1: 500-1: 3000

brain, spinal cord




Background


NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.




Images


Western blot analysis of NF-M (GB111245) at dilution of 1: 500

Immunohistochemistry of paraffin embedded mouse brain using NF-M (GB111245) at dilution of 1: 1500 (400x lens)

Immunohistochemistry of paraffin embedded mouse spinal cord using NF-M (GB111245) at dilution of 1: 1500 (400x lens)

Immunohistochemistry of paraffin embedded rat brain using NF-M (GB111245) at dilution of 1: 1500 (400x lens)

Immunohistochemistry of paraffin embedded rat spinal cord using NF-M (GB111245) at dilution of 1: 1500 (400x lens)

Immunofluorescence of paraffin embedded mouse brain using NF-M (GB111245) at dilution of 1: 500 (400x lens)

Immunofluorescence of paraffin embedded mouse spinal cord using NF-M (GB111245) at dilution of 1: 500 (400x lens)

Immunofluorescence of paraffin embedded rat brain using NF-M (GB111245) at dilution of 1: 500 (400x lens)


Immunofluorescence of paraffin embedded rat spinal cord using NF-M (GB111245) at dilution of 1: 500 (400x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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