Anti -160 kDa Neurofilament Medium Mouse mAb

價(jià)格：在線咨詢

貨號(hào)：

KT011617

產(chǎn)品規(guī)格：

100ul

Applications：

WB/IHC/IF

Species：

H/M/R,M/R

品牌：

萬(wàn)物

購(gòu)買數(shù)量：

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產(chǎn)品詳情操作說(shuō)明注意事項(xiàng)

Product Information

Description	160 kDa Neurofilament Medium mouse monoclonal antibody
Protein full name	Neurofilament medium polypeptide
Synonyms	160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nef3, Nefm, NF-M, Nfm, 150kDa medium
Immunogen	Recombinant protein corresponding to Mouse NF-M
Isotype	IgG
Purity	Affinity purification
Subcellular location	Cytoplasm, Cytoskeleton
Predicted MW.	96 kDa
Observed MW.	150 kDa
Uniprot ID	P07197, P08553, P12839

Applications

Applications	Species	Dilution	Positive Sample
WB	Human, Mouse, Rat	1: 500-1: 1000	brain, cerebral cortex, hippocampus, spinal cord
IHC/IF	Mouse, Rat	1: 500-1: 4000	brain, spinal cord, epencephalon

Background

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Images

	Western blot analysis of NF-M (GB12763) at dilution of 1: 1000
	Immunohistochemistry analysis of paraffin-embedded mouse brain using NF-M (GB12763) at dilution of 1: 4000
	Immunohistochemistry analysis of paraffin-embedded rat brain using NF-M (GB12763) at dilution of 1: 4000
	Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using NF-M (GB12763) at dilution of 1: 4000
	Immunohistochemistry analysis of paraffin-embedded rat spinal cord using NF-M (GB12763) at dilution of 1: 4000
	Immunohistochemistry analysis of paraffin-embedded mouse epencephalon using NF-M (GB12763) at dilution of 1: 4000
	Immunohistochemistry analysis of paraffin-embedded rat epencephalon using NF-M (GB12763) at dilution of 1: 4000
	Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using NF-M (GB12763) at dilution of 1: 1000
	Immunofluorescent analysis of paraformaldehyde-fixed rat brain using NF-M (GB12763) at dilution of 1: 1000
	Immunofluorescent analysis of paraformaldehyde-fixed mouse spinal cord using NF-M (GB12763) at dilution of 1: 1000
	Immunofluorescent analysis of paraformaldehyde-fixed rat spinal cord using NF-M (GB12763) at dilution of 1: 1000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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