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Anti -beta III Tubulin Mouse mAb

價格: 在線咨詢
貨號:
KT011602
產(chǎn)品規(guī)格:
100ul
Applications:
WB/ICC/IF
Species:
H/M/R,M
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

beta III Tubulin mouse monoclonal antibody

Protein full name

Tubulin beta-3 chain

Synonyms

TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III, β3- tubulin

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse β3- tubulin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

55 kDa

Observed MW.

55 kDa

Uniprot ID

Q9ERD7, Q4QRB4



Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1:1000-1:2000

cerebral cortex, hippocampus, brain

ICC/IF

Mouse

1: 500-1: 1000

Neuro-2a







Background


Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response.This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.




Images



Western blot analysis of β-III-Tubulin (GB12139) at dilution of 1: 1000

Immunofluorescence analysis of mouse Neuro-2a cells using β3- tubulin (GB12139)

at dilution of 1: 1000

A: DAPI nuclear stain ( blue )

B: Anti-β3- tubulin ( red )

C: Merge




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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