Anti -MCP1 Rabbit pAb

價(jià)格：在線咨詢

貨號(hào)：

DRF101717

產(chǎn)品規(guī)格：

100 μL

Applications：

Species：

品牌：

萬(wàn)物

購(gòu)買(mǎi)數(shù)量：

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產(chǎn)品詳情操作說(shuō)明注意事項(xiàng)

Product Information

Description	MCP1 rabbit polyclonal
Protein full name	C-C motif chemokine 2
Synonyms	CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, C-C motif chemokine ligand 2, MCP-1
Immunogen	KLH conjugated Synthetic peptide corresponding to Human MCP1
Isotype	IgG
Purity	Affinity purification
Predicted MW.	11 kDa
Observed MW.	11 kDa
Uniprot ID	P13500

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human	1: 500-1: 1000	THP-1

Background

Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or β) chemokines . The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis , rheumatoid arthritis and atherosclerosis . MCP-1 may also contribute to tumor progression and angiogenesis . Signaling by MCP-1 is mediated by the G-protein coupled receptor CCR2 .

Images

Western blot analysis of MCP1 (GB113239)

at dilution of 1: 1000

Lane 1: THP-1 untreated

Lane 2: THP-1 treated with 80nM TPA for 24h

Lane 3: THP-1 treated with 80nM TPA for 24h, then treated with 100ng/ml lipopolysaccharide (LPS) for 6h, then with 300ng/ml Brefeldin A added after 3 hours, whole cell lysate

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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